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NOTE: These instructions are quite old and describe how to install GBrowse 1.X. Since Mac OS X is very 'unix-y', once libgd installed, GBrowse2 can be installed just like it would on any other unix like system. A libgd installer for Leopard and Snow Leopard can be obtained from sourceforge
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GBrowse runs well on both PowerPC and Intel Macs running OS X. Theseinstructions describe how to install GBrowse on theseplatforms.
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Note: One of the 2009 security updates from Apple caused problems for Perl, which affect cpan and installation of Perl packages. See: Fixing Perl on OSX
- 2Prerequisites
You can download an installer for libGD for either Leopard or Snow Leopard from the GMOD SourceForge site (it's possible that the Snow Leopard installer will work for newer Mac OS systems--I haven't had a chance to check).
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You MUST have the Mac OS developer tools installed in order to install GBrowse on a Mac, since make is required. See step 1 of The Manual Way section below for more information.
GBrowse has a number of prerequisites, including BioPerl, GD (which requires libgd), and theMySQL database. You can install these prerequisites using the Finkprecompiled binary package manager, or from source code.
If you are using MySQL databases, GBrowse also requires the Perl modules to talk to MySQL, DBI and DBD::mysql, which are often not present in the Perl installations on new Macs. These can be installed using CPAN. As of 8-1-2007, there are some problems with the CPAN installers for DBD::mysql on the Mac.
The Fink Way
Make sure you have installed the most recent version of Fink, available from finkproject.org. Configure fink and run
fink selfupdate
Open up a command window, and install the following Fink packages:
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If you use fink to install any perl modules (for instance,
fink install dbi-pm586
will install DBI.pm), you should modify your Apache configuration file, httpd.conf (Note, in Mac OSX the file is located at /etc/apache2/httpd.conf). In that file, find and uncomment (that is, remove the '#' symbols) these two lines:and add this line near the bottom of the file:
Now restart the webserver either via the system preferences panel for network services, or via this command:
You do not need to install BioPerl, GD, or any other Perl packages using fink. You can install GD, BioPerl, BioGraphics and GBrowse with the cpan shell:
Currently this fails GD test 10, but that functionality isn't needed for GBrowse, so it is safe to force.
Which will complete the installation of GBrowse2. For installing GBrowse 1, go directly to Installing GBrowse using the net installer.
The MacPorts Way
Installing GBrowse using MacPorts is very similar to using Fink, with one caveat after installing libgd.
Make sure you have installed the most recent version of MacPorts, available from MacPorts install page. Configure port and run
port selfupdate
.Open up a command window, and install the following MacPorts packages:
This will install MANY programs (more than fink), including Perl and several Xorg packages. Since MacPorts modifies your $PATH variable to search the MacPorts directories first for any application, using perl and cpan as below without modifying $PATH will result in the MacPorts-installed perl to install perl modules for use with that perl. The unfortunate result of this is that the system installed Apache web server doesn't know anything about this perl, so GBrowse won't work. The easiest solution to this problem is to fix the $PATH variable before continuing. To do this, edit the .profile file in your home directory, and find the line that looks like this:
and replace it with
and save the file. Then to make your current terminal aware of the changes, you need to 'source' the profile:
or close your current terminal window and open a new one.
You do not need to install BioPerl, GD, or any other Perl packages using MacPorts. You can install GD, BioPerl, BioGraphics and GBrowse with the cpan shell:
Currently this fails GD test 10, but that functionality isn't needed for GBrowse, so it is safe to force.
Which will complete the installation of GBrowse2. For installing GBrowse 1, go directly to Installing GBrowse using the net installer.
The Manual Way
1. Make sure you have installed Mac OS X Developer tools that come on the distribution CD. Include X11 and the X11 SDK in the tools you install. To confirm that you have the tools, open a command window and type:
If any of these commands returns with the error message 'command not found,' then you should (re)install the developer tools.
2. Install MySQL (optional; only if you want to run a mysql backend)
Go to http://www.mysql.org/downloads/mysql/5.0.html#macosx-dmg, download and run the appropriate OSX installer for your version of the operating system. Note that there are separate installers for OS X versions 10.3 and upward on PowerPC 32-bit, 64-bit and Intel platforms. You may need to take care here. Joan Pontius reports that you must use the 32-bit MySQL server and DBD::mysql version 2.9007 in order for the Perl that is preinstalled on MacOS X systems to work nicely with MySQL. You can get the correct DBD::mysql version at CPAN.
3. Install libgd
This is where many people get stuck because MacOSX is ships with an older version of libgd that does not work properly with GBrowse. To add to the confusion, OSX 10.3 and earlier has older developer tools that can't build the new libgd correctly.
Worry not; just follow the recipe.
- 1.Make sure your PATH environment variable includes /usr/local/bin by running the command:
- If you do not see </tt>/usr/local/bin</tt> listed, either create or edit a file called .bash_login in your home folder (note the leading '.', which hides this file from directory listings), and add the following line to the bottom:
- 2.Make sure that you have at least version 2.58 of the autoconf tool installed. Run the command:
- This will print out the version number. It must be 2.58 or higher. If the version is too low, then upgrade autoconf like this:
- Download version 2.58 or higher from http://ftp.gnu.org/gnu/autoconf/. It's best to use 2.58 because it is known to work.
- Unpack autoconf, enter the distribution directory and type:
- 3.Install libpng
- Get the latest libpng from libpng.org. Look for the version 'with config script.'
- Unpack libpng, enter the distribution directory and type:
- 4.Install libgd
- Get the latest libgd from http://www.libgd.org.
- Unpack libgd, enter the distribution directory and type:
- If 'make' fails with errors about not being able to compile gdft.c, then reconfigure with the following commands:
- Do not try to fix this problem by reinstalling freetype unless you are very brave; you will likely to make things worse. Freetype support is not needed for GBrowse.
Whether you installed the non-Perl prerequisites with Fink ormanually, you will now use the GBrowse net installer to installBioPerl, GD and other perl-based prerequisites. You will need aworking Internet connection for this step.
Download the gbrowse_netinstall.pl script from theGBrowse distribution. Run the following command as the root useror using 'sudo':
See the main install page for a description of command line options for the netinstall script.
This will install the correct versions of GD, BioPerl, and all dependencies. During the process you will be asked to respond to various questions. It is generally safe to choose the defaults with one major exception: if you installed libgd with fink (ie, gd2), you need to answer the question about the location of libgd with '/sw/lib' instead of the default.
If this installer fails, it may be because the perl module LWP::Simple is not present. To install it, execute the command:
and then rerun the GBrowse net install script.
You may need to turn on the local web server if it isn't activated already:
You may also wish to adjust your firewall settings to control access to your machine's web server from the Internet.
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Open http://localhost/gbrowse and click the link for the example yeast chromosome 1 database.
Optional; only if you want to run the Mysql backend
If you have Mysql running and wish to take advantage of the GBrowse Mysql interface, then you will need to install the Perl DBD::mysql driver.
From the command line, type:
If you get a lot of errors about not having read/write privileges on the test database, download the DBD::mysql package from CPAN, unpack it, and follow the directions for building it manually.
There is one other issue to take care of. The bp_bulk_load_gff.pl file, which is used to load gff files into a mysql database is located in /usr/local/bin/. The script uses /usr/tmp as the temporary directory; but this doesn't exist on the Mac. Edit line 347 of bp_bulk_load_gff.pl to look like this:
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Lincoln D. Stein <[email protected]> 20:24, 16 April 2007 (EDT) Adapted from instructions contributedby Yair Benita <[email protected]>.
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